DOI: http://dx.doi.org/10.18203/2394-6040.ijcmph20214551

Anti-inflammatory activity of acacia catechu-bark aqueous solution in aspirin induced gastric ulcer in rodents

Uzma Waseem, Syeda Rizwana Jafri, Sarah Khalid, Fauzia Qureshi, Nadia Majeed, Ursula Akif

Abstract


Background: Aspirin is amongst the most widely used drugs and has many adverse effects on gastric mucosa. Anti-inflammatory properties of Acacia catechu have been established already. The objective was to evaluate the histopathological changes induced by aspirin in the stomach of albino rats and to assess the protective effect of different doses of Acacia catechu.

Methods: An experimental study was carried out in Postgraduate Medical Institute, Lahore for 21 days. Forty-eight adult albino rats, both males and females, were divided into four groups A, B, C and D randomly; each comprising of 12 rats. Group A was control, group B was given aspirin 100 mg/kg and group C and D were given aspirin 100 mg/kg along with Acacia catechu 250 mg/kg and 500 mg/kg respectively by oral route. The rats from individual group were sacrificed on 3rd day, 7th day and 14th day and stomachs were examined under light microscope to observe the inflammatory cells infiltration.

Results: Gross and microscopic findings on days 3, 7 and 14 were similar. Control groups A1, A2 and A3 showed normal healthy gastric mucosa and the least number of inflammatory cells. In group B, aspirin produced ulcerations and linear breaks; with highest inflammatory infiltrates. On microscopic examination, numerous inflammatory cells were noted. Group C and D rats had minimum ulcer index and fewer inflammatory cells.

Conclusions: Acacia catechu has protective role against gastric injury by inhibiting inflammation.


Keywords


Acacia catechu, Anti-inflammatory, Rodents, Ulcer

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References


Barbouchi M, Elamrani K, El Idrissi M. A comparative study on phytochemical screening, quantification of phenolic contents and antioxidant properties of different solvent extracts from various parts of Pistacia lentiscus L. J King Saud Univ Sci. 2020;32(1):302-6.

Khare B, Dubey N, Sharma A. antiulcer activity of controlled release formulation containing aqueous extract of acacia catechu willd on rodent models. Int J Curr Pharm Res. 2018;10(5):25-31.

Alam G, Singh MP, Singh A, Patel R. Investigation of anthelmintic, anti-inflammatory activity of leaves extract of Acacia catechu Willd. J Pharm Res. 2012;5(5):2587-9.

Rashid M, Shamsi S, zaman R, Ilahi A. Kath (Acacia catechu): an overarching envelop of traditional and modern update. Int J Curr Trends Pharm Res. 2015;3(5):1007-12.

Deepak SK, Sreenivas Reddy GR. Mohiuddin M, Pranav Kumar AVR, Sai Krishna DM, Potbhare MS. Investigation of poly-herbal aqueous extract for potential anti-ulcer activity. Int J Pharm Res. 2013;3(3):53-8.

Alambayan J, Vats M, Sardana S, Sehrawat R. Evaluation of antiulcer activity of roots of Acacia catechu Willd. (Mimosoideae). J Pharmacog Phytochem. 2015;3(5):79-84.

Alam S, Hussain MS, Reddy MK, Reddy M, Gupta RK. Antiulcer and antioxidant potential of Zizyphus jujuba Mill root extract in aspirin and ethanol induced gastric ulcers. IJP. 2016;8:287-93.

Thendral T, Lakshmi T. Antifungal activity of Acacia catechu bark extract against dermatophytes: an in vitro study. J Adv Pharm Edu Res. 2017;7:25-7.

Thakur AV, Ambwani S, Ambwani TK. Preliminary phytochemical screening and GC-MS analysis of leaf extract of Acacia catechu (Lf) Willd. Int J Herb Med. 2018;6(2):81-5.

Park JU, Kang JH, Rahman MA, Hussain A, Cho JS, Lee YI. Gastroprotective effects of plants extracts on gastric mucosal injury in experimental sprague-dawley rats. BioMed Res Int. 2019;2019:1-11.

Escobedo-Hinojosa WI, Gomez-Chang E, García-Martínez K, Guerrero Alquicira R, Cardoso-Taketa A, Romero I. Gastroprotective mechanism and ulcer resolution effect of Cyrtocarpa procera methanolic extract on ethanol-induced gastric injury. Evid Based Complemen Altern Med. 2018;2018.

Sattar A, Abdo A, Mushtaq MN, Anjum I, Anjum A. Evaluation of gastro-protective activity of Myristica fragrans on ethanol-induced ulcer in albino rats. Anais da Academia Brasileira de Ciências. 2019;91(2):1-8.

Tong Y, Jiang Y, Chen X, Li X, Wang P, Jin Y, et al. Extraction, enrichment, and quantification of main antioxidant aglycones of flavonoids and tannins from Melastoma dodecandrum Lour.: Guided by UPLC-ESI-MS/MS. J Chem. 2019;2019.

Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011;89(3):217-33.

Pisoschi AM, Pop A, Cimpeanu C, Predoi G. Antioxidant capacity determination in plants and plant-derived products: an review. Oxidat Med Cell Long. 2016;2016.

Kato S, Suzuki K, Ukawa H, Komoike Y, Takeuchi K. Low gastric toxicity of nitric oxide-releasing aspirin, NCX-4016, in rats with cirrhosis and arthritis. Dig Dis Sci. 2001;46(8):1690-9.

Ray D, Sharatchandra KH, Thokchom IS. Antipyretic, antidiarrheal, hypoglycaemic and hepatoprotective activities of ethyl acetate extract of Acacia catechu Willd. in albino rats. Indian J Pharmacol. 2006;38(6):408-13.

Margadent C, Sonnenberg A. Integrin–TGF-β crosstalk in fibrosis, cancer and wound healing. EMBO Rep. 2010;11(2):97-105.

Martin P, Leibovich SJ. Inflammatory cells during wound repair: the good, the bad and the ugly. Trends Cell Biol. 2005;15:599-607.

Robert F, Diegelmann RF, Evans MC. Wound healing of acute, fibrotic and delayed healing. Front Biosci. 2004;9:283-9.

Eming SA, Krieg T, Davidson JM. Inflammation in wound repair: molecular and cellular mechanisms. J Investig Dermatol. 2007;127(3):514-25.

Jainu M, Devi CSS. Gastroprotective action of Cissus quadrangularis extract against NSAID induced gastric ulcer: role of proinflammatory cytokines and oxidative damage. Chem Biol Interact. 2006;161(3): 262-70.

Altavilla D, Squadrito F, Minutoli L. Flavocoxid, a dual inhibitor of cyclooxygenase and 5-lipoxygenase, blunts pro-inflammatory phenotype activation in endotoxin-stimulated macrophages. Br J Pharmacol. 2009;157(8):1410-8.

Lewis C, Murdoch C. Macrophage responses to hypoxia: implications for tumor progression and anti-cancer therapies. Am J Path. 2005;167(3):627-35.

Derin N, Izgut-Uysal VN, Agac A, Aliciguzel Y, Demir N. L-Carnitine protects gastric mucosa by decreasing ischemia- reperfusion induced lipid peroxidation. J Physiol Pharmacol. 2004;55(3):595-606.

Souza MH, Lemos HP, Oliveira RB, Cunha FQ. Gastric damage and granulocyte infiltration induced by indomethacin in tumour necrosis factor receptor 1 (TNF-R1) or inducible nitric oxide synthase (iNOS) deficient mice. Gut. 2004;53(6):791-6.

Burnett B, Jia Q, Zhao Y, Levy R. A medicinal extract of Scutellaria baicalensis and Acacia catechu acts as a dual inhibitor of cyclooxygenase and 5-lipoxygenase to reduce inflammation. J Med Food. 2007;10(3):442-51.

Newton PM, Watson JA, Wolowacz RG, Wood EJ. Macrophages restrain contraction of an in vitro wound healing model. Inflamm. 2004;28(4):207-14.

Kokura S, Wolf RE, Yoshikawa T, Granger DN, Aw TY. T-lymphocyte-derived tumor necrosis factor exacerbates anoxia-reoxygenation-induced neutrophil-endothelial cell adhesion. Circ Res. 2000;86:205-13.

Tang XL, Liu JX, Dong W, Li P, Li L, Zheng YQ. et al. Intervention effect of quercetin on inflammatory secretion of cardiac fibroblasts. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica. 2014;39(12):2314-7.

Arjmandi BH, Ormsbee LT, Elam ML. A combination of Scutellaria baicalensis and Acacia catechu extracts for short-term symptomatic relief of joint discomfort associated with osteoarthritis of the knee. J Med Food. 2014;17:707-13.

Muruganandan S, Srinivasan K, Chandra S, Tandan SK, Lal J, Raviprakash V. Anti-inflammatory activity of Syzygium cumini bark. Fitoterapia. 2001;72(4):369-75.